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prsshrna control tr30012 plasmids  (OriGene)


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    Structured Review

    OriGene prsshrna control tr30012 plasmids
    Prsshrna Control Tr30012 Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+shrna+prs+plasmid/Scrambled+shRNA+control+in+pRS+shRNA+Vector/pm37596321-200-15-22
    Average 95 stars, based on 176 article reviews
    prsshrna control tr30012 plasmids - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    shRNA:

    Article Title: Sphingosine-1-phosphate receptor-3 signaling up-regulates epidermal growth factor receptor and enhances epidermal growth factor receptor-mediated carcinogenic activities in cultured lung adenocarcinoma cells
    Article Snippet: To specifically knockdown the S1P 3 receptor, cells were transfected with 1 μ g of shRNA vector constructs (Origene) using Lipofectamine TM 2000 (Invitrogen) according to manufacturer’s instructions. .. The shRNA constructs (5′-GCTTC ATCGT CTTGG AGAAC CTGAT GGTT-3′) used to silence the S1P 3 receptor and the negative control shRNA pRS plasmid were purchased from Origene (Rockville, MD). ..

    Article Title: ?-Catenin/NPRAP: A New Member of the Glycogen Synthase Kinase-3? Signaling Complex That Promotes ?-catenin Turnover in Neurons
    Article Snippet: Cells were transfected using the Lipofectamine 2000 method with the following plasmids; HA-GSK-3β-WT (He et al., 1995), HA-GSK-3β-S9A, constituitively active mutant (CA) (Stambolic et al., 1994), and HA-GSK3β-K85A, kinase dead mutant (KD) were kindly provided by Dr. Jim Woodget through Addgene. .. Primary cortical neurons were transfected with a set of small-hairpin (shRNA) corresponding to rat δ-catenin mRNA. shRNA constructs were designed and synthesized by (Origene) as follows: 5′-CTGGTCACAGGAGTCCTTTGGAACCTCTC-3′ and control shRNA pRS plasmid was used as a negative control. .. The Amaxa electroporation method was used for transfections of control and shRNA plasmids.

    Construct:

    Article Title: Sphingosine-1-phosphate receptor-3 signaling up-regulates epidermal growth factor receptor and enhances epidermal growth factor receptor-mediated carcinogenic activities in cultured lung adenocarcinoma cells
    Article Snippet: To specifically knockdown the S1P 3 receptor, cells were transfected with 1 μ g of shRNA vector constructs (Origene) using Lipofectamine TM 2000 (Invitrogen) according to manufacturer’s instructions. .. The shRNA constructs (5′-GCTTC ATCGT CTTGG AGAAC CTGAT GGTT-3′) used to silence the S1P 3 receptor and the negative control shRNA pRS plasmid were purchased from Origene (Rockville, MD). ..

    Article Title: ?-Catenin/NPRAP: A New Member of the Glycogen Synthase Kinase-3? Signaling Complex That Promotes ?-catenin Turnover in Neurons
    Article Snippet: Cells were transfected using the Lipofectamine 2000 method with the following plasmids; HA-GSK-3β-WT (He et al., 1995), HA-GSK-3β-S9A, constituitively active mutant (CA) (Stambolic et al., 1994), and HA-GSK3β-K85A, kinase dead mutant (KD) were kindly provided by Dr. Jim Woodget through Addgene. .. Primary cortical neurons were transfected with a set of small-hairpin (shRNA) corresponding to rat δ-catenin mRNA. shRNA constructs were designed and synthesized by (Origene) as follows: 5′-CTGGTCACAGGAGTCCTTTGGAACCTCTC-3′ and control shRNA pRS plasmid was used as a negative control. .. The Amaxa electroporation method was used for transfections of control and shRNA plasmids.

    Negative Control:

    Article Title: Sphingosine-1-phosphate receptor-3 signaling up-regulates epidermal growth factor receptor and enhances epidermal growth factor receptor-mediated carcinogenic activities in cultured lung adenocarcinoma cells
    Article Snippet: To specifically knockdown the S1P 3 receptor, cells were transfected with 1 μ g of shRNA vector constructs (Origene) using Lipofectamine TM 2000 (Invitrogen) according to manufacturer’s instructions. .. The shRNA constructs (5′-GCTTC ATCGT CTTGG AGAAC CTGAT GGTT-3′) used to silence the S1P 3 receptor and the negative control shRNA pRS plasmid were purchased from Origene (Rockville, MD). ..

    Article Title: ?-Catenin/NPRAP: A New Member of the Glycogen Synthase Kinase-3? Signaling Complex That Promotes ?-catenin Turnover in Neurons
    Article Snippet: Cells were transfected using the Lipofectamine 2000 method with the following plasmids; HA-GSK-3β-WT (He et al., 1995), HA-GSK-3β-S9A, constituitively active mutant (CA) (Stambolic et al., 1994), and HA-GSK3β-K85A, kinase dead mutant (KD) were kindly provided by Dr. Jim Woodget through Addgene. .. Primary cortical neurons were transfected with a set of small-hairpin (shRNA) corresponding to rat δ-catenin mRNA. shRNA constructs were designed and synthesized by (Origene) as follows: 5′-CTGGTCACAGGAGTCCTTTGGAACCTCTC-3′ and control shRNA pRS plasmid was used as a negative control. .. The Amaxa electroporation method was used for transfections of control and shRNA plasmids.

    Plasmid Preparation:

    Article Title: Sphingosine-1-phosphate receptor-3 signaling up-regulates epidermal growth factor receptor and enhances epidermal growth factor receptor-mediated carcinogenic activities in cultured lung adenocarcinoma cells
    Article Snippet: To specifically knockdown the S1P 3 receptor, cells were transfected with 1 μ g of shRNA vector constructs (Origene) using Lipofectamine TM 2000 (Invitrogen) according to manufacturer’s instructions. .. The shRNA constructs (5′-GCTTC ATCGT CTTGG AGAAC CTGAT GGTT-3′) used to silence the S1P 3 receptor and the negative control shRNA pRS plasmid were purchased from Origene (Rockville, MD). ..

    Article Title: ?-Catenin/NPRAP: A New Member of the Glycogen Synthase Kinase-3? Signaling Complex That Promotes ?-catenin Turnover in Neurons
    Article Snippet: Cells were transfected using the Lipofectamine 2000 method with the following plasmids; HA-GSK-3β-WT (He et al., 1995), HA-GSK-3β-S9A, constituitively active mutant (CA) (Stambolic et al., 1994), and HA-GSK3β-K85A, kinase dead mutant (KD) were kindly provided by Dr. Jim Woodget through Addgene. .. Primary cortical neurons were transfected with a set of small-hairpin (shRNA) corresponding to rat δ-catenin mRNA. shRNA constructs were designed and synthesized by (Origene) as follows: 5′-CTGGTCACAGGAGTCCTTTGGAACCTCTC-3′ and control shRNA pRS plasmid was used as a negative control. .. The Amaxa electroporation method was used for transfections of control and shRNA plasmids.

    Transfection:

    Article Title: ?-Catenin/NPRAP: A New Member of the Glycogen Synthase Kinase-3? Signaling Complex That Promotes ?-catenin Turnover in Neurons
    Article Snippet: Cells were transfected using the Lipofectamine 2000 method with the following plasmids; HA-GSK-3β-WT (He et al., 1995), HA-GSK-3β-S9A, constituitively active mutant (CA) (Stambolic et al., 1994), and HA-GSK3β-K85A, kinase dead mutant (KD) were kindly provided by Dr. Jim Woodget through Addgene. .. Primary cortical neurons were transfected with a set of small-hairpin (shRNA) corresponding to rat δ-catenin mRNA. shRNA constructs were designed and synthesized by (Origene) as follows: 5′-CTGGTCACAGGAGTCCTTTGGAACCTCTC-3′ and control shRNA pRS plasmid was used as a negative control. .. The Amaxa electroporation method was used for transfections of control and shRNA plasmids.

    Synthesized:

    Article Title: ?-Catenin/NPRAP: A New Member of the Glycogen Synthase Kinase-3? Signaling Complex That Promotes ?-catenin Turnover in Neurons
    Article Snippet: Cells were transfected using the Lipofectamine 2000 method with the following plasmids; HA-GSK-3β-WT (He et al., 1995), HA-GSK-3β-S9A, constituitively active mutant (CA) (Stambolic et al., 1994), and HA-GSK3β-K85A, kinase dead mutant (KD) were kindly provided by Dr. Jim Woodget through Addgene. .. Primary cortical neurons were transfected with a set of small-hairpin (shRNA) corresponding to rat δ-catenin mRNA. shRNA constructs were designed and synthesized by (Origene) as follows: 5′-CTGGTCACAGGAGTCCTTTGGAACCTCTC-3′ and control shRNA pRS plasmid was used as a negative control. .. The Amaxa electroporation method was used for transfections of control and shRNA plasmids.

    Control:

    Article Title: ?-Catenin/NPRAP: A New Member of the Glycogen Synthase Kinase-3? Signaling Complex That Promotes ?-catenin Turnover in Neurons
    Article Snippet: Cells were transfected using the Lipofectamine 2000 method with the following plasmids; HA-GSK-3β-WT (He et al., 1995), HA-GSK-3β-S9A, constituitively active mutant (CA) (Stambolic et al., 1994), and HA-GSK3β-K85A, kinase dead mutant (KD) were kindly provided by Dr. Jim Woodget through Addgene. .. Primary cortical neurons were transfected with a set of small-hairpin (shRNA) corresponding to rat δ-catenin mRNA. shRNA constructs were designed and synthesized by (Origene) as follows: 5′-CTGGTCACAGGAGTCCTTTGGAACCTCTC-3′ and control shRNA pRS plasmid was used as a negative control. .. The Amaxa electroporation method was used for transfections of control and shRNA plasmids.



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    Figure 5. Effect of ADM2 on the expression of eNOS, MMP2, MMP9, and protein S-nitrosylation in HTR-8/SVneo cells. A, Agarose gel picture showing <t>that</t> <t>knockdown</t> of ADM2 mRNA expression in HTR-8/SVneo cells results in decreased expression of eNOS, MMP2, and MMP9 mRNA compared with the control (sh scramble). B, A zymogram gel picture showing an effect of ADM2 (108 M) supplementation on the gelatinase activity of MMP2 and MMP9 in HTR-8/SVneo cells in presence or absence of L-NAME (104 M). Bar graph representing mean SEM of the fold change shows that addition of ADM2 increases the gelatinase activity of MMP2 and MMP9 in HTR-8/SVneo cells, which is inhibited in the presence of L-NAME. *, Significant difference compared with the control (n 3; P .05). C, Immunofluorescent staining showing effect of ADM2 on the eNOS, MMP2, and MMP9 immunoreactivity in HTR-8/SVneo cells cultured on inserts coated with Matrigel. As shown, addition of ADM2 (108 M) increases the immunoreactivity of eNOS, MMP2, and MMP9 in the HTR-8/SVneo cells that invaded the Matrigel compared with the untreated controls. 4, 6-Diamidino-2-phenylindole (DAPI) was used for blue nuclear staining and IgG was used as a negative control. Bar graph shows ADM2 immunostaining relative to DAPI. Data are presented as mean SEM. *, P .05 (magnification, 200, n 3). D, Western blot showing that silencing of ADM2 gene using ADM2-specific <t>shRNA</t> transfection decreases the expression of S-nitrosylated proteins in HTR-8/ SVneo cells compared with the cells transfected with scramble shRNA. As shown, the decrease is more prominent in proteins with weight of 70 kDa or greater. The blot is representative of three experiments.
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    Image Search Results


    Figure 5. Effect of ADM2 on the expression of eNOS, MMP2, MMP9, and protein S-nitrosylation in HTR-8/SVneo cells. A, Agarose gel picture showing that knockdown of ADM2 mRNA expression in HTR-8/SVneo cells results in decreased expression of eNOS, MMP2, and MMP9 mRNA compared with the control (sh scramble). B, A zymogram gel picture showing an effect of ADM2 (108 M) supplementation on the gelatinase activity of MMP2 and MMP9 in HTR-8/SVneo cells in presence or absence of L-NAME (104 M). Bar graph representing mean SEM of the fold change shows that addition of ADM2 increases the gelatinase activity of MMP2 and MMP9 in HTR-8/SVneo cells, which is inhibited in the presence of L-NAME. *, Significant difference compared with the control (n 3; P .05). C, Immunofluorescent staining showing effect of ADM2 on the eNOS, MMP2, and MMP9 immunoreactivity in HTR-8/SVneo cells cultured on inserts coated with Matrigel. As shown, addition of ADM2 (108 M) increases the immunoreactivity of eNOS, MMP2, and MMP9 in the HTR-8/SVneo cells that invaded the Matrigel compared with the untreated controls. 4, 6-Diamidino-2-phenylindole (DAPI) was used for blue nuclear staining and IgG was used as a negative control. Bar graph shows ADM2 immunostaining relative to DAPI. Data are presented as mean SEM. *, P .05 (magnification, 200, n 3). D, Western blot showing that silencing of ADM2 gene using ADM2-specific shRNA transfection decreases the expression of S-nitrosylated proteins in HTR-8/ SVneo cells compared with the cells transfected with scramble shRNA. As shown, the decrease is more prominent in proteins with weight of 70 kDa or greater. The blot is representative of three experiments.

    Journal: The Journal of clinical endocrinology and metabolism

    Article Title: Adrenomedullin2 (ADM2)/Intermedin (IMD): A Potential Role in the Pathophysiology of Preeclampsia.

    doi: 10.1210/jc.2016-1333

    Figure Lengend Snippet: Figure 5. Effect of ADM2 on the expression of eNOS, MMP2, MMP9, and protein S-nitrosylation in HTR-8/SVneo cells. A, Agarose gel picture showing that knockdown of ADM2 mRNA expression in HTR-8/SVneo cells results in decreased expression of eNOS, MMP2, and MMP9 mRNA compared with the control (sh scramble). B, A zymogram gel picture showing an effect of ADM2 (108 M) supplementation on the gelatinase activity of MMP2 and MMP9 in HTR-8/SVneo cells in presence or absence of L-NAME (104 M). Bar graph representing mean SEM of the fold change shows that addition of ADM2 increases the gelatinase activity of MMP2 and MMP9 in HTR-8/SVneo cells, which is inhibited in the presence of L-NAME. *, Significant difference compared with the control (n 3; P .05). C, Immunofluorescent staining showing effect of ADM2 on the eNOS, MMP2, and MMP9 immunoreactivity in HTR-8/SVneo cells cultured on inserts coated with Matrigel. As shown, addition of ADM2 (108 M) increases the immunoreactivity of eNOS, MMP2, and MMP9 in the HTR-8/SVneo cells that invaded the Matrigel compared with the untreated controls. 4, 6-Diamidino-2-phenylindole (DAPI) was used for blue nuclear staining and IgG was used as a negative control. Bar graph shows ADM2 immunostaining relative to DAPI. Data are presented as mean SEM. *, P .05 (magnification, 200, n 3). D, Western blot showing that silencing of ADM2 gene using ADM2-specific shRNA transfection decreases the expression of S-nitrosylated proteins in HTR-8/ SVneo cells compared with the cells transfected with scramble shRNA. As shown, the decrease is more prominent in proteins with weight of 70 kDa or greater. The blot is representative of three experiments.

    Article Snippet: The knockdown transfection was performed with four ADM2 shRNAs and one scramble control shRNA plasmid (OriGene) using Lipofectamine 2000 following the manufacturer’s protocol (Invitrogen).

    Techniques: Expressing, Agarose Gel Electrophoresis, Knockdown, Control, Activity Assay, Staining, Cell Culture, Negative Control, Immunostaining, Western Blot, shRNA, Transfection